Greenup is a city in Greenup County, Kentucky, at the confluence of the Ohio and Little Sandy Rivers. The population was 1,198 at the 2000 census. It is the county seat of Greenup County. Greenup is named in honor of Christopher Greenup. Greenup is a part of the Huntington-Ashland, WV-KY-OH Metropolitan Statistical Area (MSA). As of the 2000 census, the MSA had a population of 288,649.