Sanford is a city in and the county seat of Seminole County, Florida, United States. The population was 38,291 at the 2000 census. As of 2006, the population recorded by the U.S. Census Bureau was 49,124. An older agricultural and resort area, Sanford is home to the Delta Connection Academy, Seminole State College of Florida and the Central Florida Zoo and Botanical Gardens. The city is situated beside Lake Monroe and the St. Johns River. It is part of the Orlando–Kissimmee Metropolitan Statistical Area.